Beschreibung
InhaltsangabePart I: General1. Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations Alec W.M. Simpson 2. Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2 Anish Patel, Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, and David G. LambertPart II: Specialist Measurement Systems3. Confocal Microscopy: Theory and Applications for Cellular Signaling Stephen C. Tovey, Paul J. Brighton, Edward T.W. Bampton, Yan Huang, and Gary B. Willars4. Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer Ian C.B. Marshall, Izzy Boyfield, and Shaun McNulty5. Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR®) Ian C.B. Marshall, Davina E. Owen, and Shaun McNulty6. Ratiometric [Ca2+]i Measurements in Adherent Cell-lines Using the NOVOstar Microplate Reader Benjamin D. Hunt and David G. LambertPart III: Measurement of Ca2+ Channel Activity7. Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones Jon Brown, Atticus H. Hainsworth, Alessandro Stefani, and Andrew D. Randall8. Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells Richard D. RainbowPart IV: Measurement of Ins(1,4,5)P3 and Ca2+ Release Form Intracellular Stores9. Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol Labeling Protocols in Permeabilized Cells Alison Skippen, Philip Swigart, and Shamshad Cockcroft 10. Single Cell Imaging Techniques for the Real-Time Detection of IP3 in Live Cells Carl P. Nelson11. Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay Darren SmartPart V: Specialist Measurement Techniques 12. Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry Hideo Kanaide and Katsuya Hirano13. Calcium Measurements from Whole Heart Using Rhod-2 Bum-Rak Choi14. Measurement of Changes in Endothelial and Smooth Muscle Ca2+ in Pressurized Arteries Kim A. Dora and Michael A. Hill15. Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration John G. McCarron, Marnie L. Olson, Susan Chalmers, and John M. Girkin16. Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations Raul Martinez-Zaguilan, Linda S. Tompkins, Robert J. Gillies, and Ronald M. Lynch17. Measurements of Ca2+ Concentration with Recombinant Targeted Luminescent Probes Denis Ottolini,Tito Calì, and Marisa Brini18. Chimeric G-Proteins in Fluorimetric Calcium Assays: Experience with Opioid Receptors Valeria Camarda and Girolamo Calo 19. Compartmentalizing Genetically-Encoded Calcium Sensors David A. Williams, Mastura Monif, and Kate L. Richardson 20. Measurement of Cytosolic-Free Ca2+ in Plant Tissue Martin R. McAinsh and Carl K.-Y. Ng21. Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1) Danuta Kosk-Kosicka
Autorenportrait
InhaltsangabePart I: General 1. Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations Alec W.M. Simpson 2. Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2 Anish Patel, Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, and David G. Lambert Part II: Specialist Measurement Systems 3. Confocal Microscopy: Theory and Applications for Cellular Signaling Stephen C. Tovey, Paul J. Brighton, Edward T.W. Bampton, Yan Huang, and Gary B. Willars 4. Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer Ian C.B. Marshall, Izzy Boyfield, and Shaun McNulty 5. Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR®) Ian C.B. Marshall, Davina E. Owen, and Shaun McNulty 6. Ratiometric [Ca2+]i Measurements in Adherent Cell-lines Using the NOVOstar Microplate Reader Benjamin D. Hunt and David G. Lambert Part III: Measurement of Ca2+ Channel Activity 7. WholeCell Patch Clamp Recording of VoltageSensitive Ca2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones Jon Brown, Atticus H. Hainsworth, Alessandro Stefani, and Andrew D. Randall 8. Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells Richard D. Rainbow Part IV: Measurement of Ins(1,4,5)P3 and Ca2+ Release Form Intracellular Stores 9. Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol Labeling Protocols in Permeabilized Cells Alison Skippen, Philip Swigart, and Shamshad Cockcroft 10. Single Cell Imaging Techniques for the Real-Time Detection of IP3 in Live Cells Carl P. Nelson 11. Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay Darren Smart Part V: Specialist Measurement Techniques 12. Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry Hideo Kanaide and Katsuya Hirano 13. Calcium Measurements from Whole Heart Using Rhod-2 Bum-Rak Choi 14. Measurement of Changes in Endothelial and Smooth Muscle Ca2+ in Pressurized Arteries Kim A. Dora and Michael A. Hill 15. Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration John G. McCarron, Marnie L. Olson, Susan Chalmers, and John M. Girkin 16. Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations Raul Martinez-Zaguilan, Linda S. Tompkins, Robert J. Gillies, and Ronald M. Lynch 17. Measurements of Ca2+ Concentration with Recombinant Targeted Luminescent Probes Denis Ottolini,Tito Calì, and Marisa Brini 18. Chimeric G-Proteins in Fluorimetric Calcium Assays: Experience with Opioid Receptors Valeria Camarda and Girolamo Calo 19. Compartmentalizing Genetically-Encoded Calcium Sensors David A. Williams, Mastura Monif, and Kate L. Richardson 20. Measurement of Cytosolic-Free Ca2+ in Plant Tissue Martin R. McAinsh and Carl K.-Y. Ng 21. Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1) Danuta Kosk-Kosicka
